Effective Laboratory Diagnosis of Parasitic Infections of the . . . The only fecal antigen test that can distinguish E histolytica from E dispar and E moshkovskii is microwell ELISA, which detects the Gal GalNAc adherence lectin of E histolytica; this test is more sensitive than stool ova and parasite examination or culture, and is rapid, taking less than two hours However, one limitation is the need for
The case for parasitological stool microscopy Bearing in mind that conventional light microscopy has low sensitivity for Cyclo-spora, adding this as a target in laboratory developed multiplex PCRs would make a lot of sense
Diagnosis of Parasitic Infections: What’s Going On? - SAGE Journals Labor-intensive methods such as microscopy still remain the mainstay of several diagnostic laboratories There is a need for more rapid tests that do not sacrifice sensitivity and that can be used in both clinical settings as well as in poor resource field settings
Improved Detection of Five Major Gastrointestinal Pathogens by Use of a . . . Microscopy in particular has disadvantages, as the detection and correct identification of parasites depend upon the experience and skills of the microscopist Also, due to intermittent shedding of protozoa the sensitivity can be low, and therefore examination of multiple samples is required ( 8 )
An Overview of the Molecular Methods in the Diagnosis of . . . Traditional laboratory diagnoses of gastrointestinal infections and enteric pathogens detection are performed by (1) culture and antibiotic susceptibility testing, (2) ova and parasite microscopy examination, and (3) antigen detection via immunoassays
Comparison of Microscopy and PCR for Detection of Intestinal Parasites . . . The sensitivity of FECT-microscopy compared to that of PCR was only 38% in terms of detection of G intestinalis Cryptosporidium was not detected by microscopy but was detected in 16 samples by PCR The higher sensitivity of PCR supports findings from other studies (1, 7, 10)
Disentangling the effects of intermittent faecal shedding and imperfect . . . Using Giardia infections as a case-study, here we illustrate a statistical-modelling approach that can be used to separately estimate parasite-shedding probability and diagnostic-test sensitivity We ran duplicate microscopy-based tests on 413 stool samples drawn from 276 children, and found that Giardia shedding probability was ~44%; that