13. Structure Determination: Nuclear Magnetic Resonance . . . The Use of NMR Spectroscopy Used to determine relative location of atoms within a molecule Most helpful spectroscopic technique in organic chemistry Related to MRI in medicine (Magnetic Resonance Imaging) Maps carbon-hydrogen framework of molecules Depends on very strong magnetic fields 13 1 Nuclear Magnetic Resonance Spectroscopy 1H or 13C nucleus spins and the internal magnetic field aligns
Slide 1 Ed 2002, 41, 3096-3129 Solid-State NMR Powder Pattern Chemical Shift Anisotropy Chemical shift is dependent on orientation of nuclei in the solid Distribution of chemical shifts Averaged to zero for isotropic tumbling Leads to extensive line-width broadening in solid-state NMR Progress in Nuclear Magnetic Resonance Spectroscopy 6 46 (2005) 1
PowerPoint Presentation The relative area under the resonances at = 4 20 and 3 50 is 2:3 TMS = 3 50, 3H = 4 20, 2H 13 7: Spin-spin splitting in 1H NMR spectroscopy protons on adjacent carbons will interact and “split” each others resonances into multiple peaks (multiplets) n + 1 rule: equivalent protons that have n equivalent protons on the adjacent carbon will be
Optical Antennas for Raman Spectroscopy - + - + Transformer 3D Taper (Optical Transformer) Au Single molecule detection requires electric field enhancement of 1014 1016 1014 1012 1010 108 106 104 102 1 Optical Antenna Grating Coupler Optical Transformer 2D Taper Au The Side View Initial Thickness Final Thickness Taper Angle Au Au The Side View Optimizing Taper Angle Power Throughput
13. Structure Determination: Nuclear Magnetic Resonance . . . * The Use of NMR Spectroscopy Used to determine relative location of atoms within a molecule Most helpful spectroscopic technique in organic chemistry Related to MRI in medicine (Magnetic Resonance Imaging) Maps carbon-hydrogen framework of molecules Depends on very strong magnetic fields * Nuclear Magnetic Resonance Spectroscopy 1H or 13C nucleus spins and the internal magnetic field
NMR spectroscopy - Louisiana Tech University Objectives Student should gain better understanding of NMR spectroscopy Student should gain experience in the acquisition, processing, and displaying NMR data Student should gain experience in interpreting NMR data in order to establish structure for unknown organic molecules Student should gain understanding in advanced 1Dimensional and 2Dimensional NMR techniques Introduction The Nobel
Slide 1 NMR Structure Determination We Need to Assign Each NOE Cross-Peak to A Specific 1H-1H Pair from Assignment Table 10 5 ppm to 7 65 ppm Assignment Table What H assignments for the protein are consistent with 10 5 ppm 7 65 ppm?