Rapid and efficient degradation of endogenous proteins To definitively test whether brain tissue is capable of degrading endogenous proteins using the dTAG system, we performed intracerebral injections of dTAGv-1 and dTAG-13 into the somatosensory cortex of Nelfb dTAG dTAG mice
dTAG System: Inducible Protein Degradation for Target Validation The dTAG system operates through a mechanism allowing inducible degradation of specific proteins Degron tags, engineered for recognition by ligands, bridge the target protein and the ubiquitin-proteasome pathway
The dTAG system for immediate and target-specific protein degradation Using dTAG, we observe an unexpected superior anti-proliferative effect of pan-BET bromodomain degradation over selective BRD4 degradation, characterize immediate effects of KRAS G12V loss on proteomic signaling, and demonstrate rapid degradation in vivo This technology platform will confer kinetic resolution to biological investigation and
The dTAG system for immediate and target-specific protein . . . - PubMed The dTAG system pairs a novel degrader of FKBP12 F36V with expression of FKBP12 F36V in-frame with a protein of interest By transgene expression or CRISPR-mediated locus-specific knock-in, we exemplify a generalizable strategy to study the immediate consequence of protein loss
Validating Targets for TPD using dTAG - Bio-Techne of the dTAG platform include the ability to tune the extent of protein knockdown by varying the Degrader dose, and the more rapid onset of action (kinetics) for studying ‘fast biology’
TAG Degradation | aTAG, dTAG, BromoTAG | Bio-Techne dTAG, aTAG and BromoTag Degraders can be used for target validation and exploration, offering an attractive alternative to genetic knockdown knockout The table below provides a comparison of TAG Degradation with commonly used genetic methods, including CRISPR Cas9 and RNA interference