Chapter 7 Assignment Flashcards | Quizlet To determine the optimum growth temperature for a new isolate, you would perform which of the following? Perform the streak plate technique, and incubate the plates at a variety of temperatures to see when it grows
Bacteriology Culture Guide | ATCC Place the plates and test tube slant cultures into an anaerobe jar with an active catalyst and a microaerophilic gas generator Gas Pak or within an incubator that can be set up for microaerophilic conditions (3-5% oxygen and 10% carbon dioxide)
LAB 4. Cultivation of Bacteria - UMD To test for the growth of numerous bacterial species on one petri dish, the plate may be streaked in sectors The streaking will not allow observation of isolated colonies, but will allow the determination of positive or negative growth
Growth and Maintenance of Escherichia coli Laboratory Strains An abbreviated list of commonly used E coli strains is given in Table 5A 4 1 with genotypes and typical uses for each strain, but a more comprehensive strain list, including gene-specific information, can be found online (see Internet References)
Bergeys Manual of Determinative Bacteriology_ Eighth -- D_ H . . . Simpler biochemical and physiological tests which are frequently useful inidentification of bacteria at the species level include oxidase, catalase, urease,nitrate reduction, H2S production, acid or gas production from sugars, tests ofamino acid metabolism, temperature range of growth, response to XaCl andantibiotic sensitivity
Bacterial Growth Curves in 96-well microplates - protocols Use your Plate Layout Template to plan your experiment Include 1-3 technical replicates of appropriate blank (s) per plate For ‘normal’ 96-well plates, use 200 ul volume For half-area 96-well plates, use 50 ul volume