Output of a Diffractometer - Chemistry Stack Exchange 3 Working with a single crystal, what is the first thing the diffractometer has access to? What is the output I see and what are the steps from this two levels of information? If I'm correct, the output might be (hkl) indexes and their intensities, but I don't know how the instrument can assign the coordinates in reciprocal space to the spots
biochemistry - How do I interpret the results of this DNA gel . . . This run was meant to be a sort of mock-forensics experiment There is DNA from the "crime scene", "suspect 1", and "suspect 2" There are 3 samples from each, one is untreated, one is digested with EcoRV, and one is digested with PstI
What causes the DNA fragments to stop moving in gel electrophoresis? Also today it is quite common to have the DNA stain already in the gel while the electrophoresis is running (instead of adding a staining solution at the end of the run) This allows to follow the DNA run in "real time"
Why is the Haber process carried out at such high temperatures? As others have pointed out, it is purely kinetics, but you may still wonder, why For a reaction to actually occur (in both directions) and thus for an equilibrium to be reached, you need to overcome the activation energy In the case of the Haber-Bosch process, this involves breaking the highly stable $\ce {N#N}$ triple bond Even with the catalysts used, the energy required to break apart
Why does the addition of heavy water cause OH and NH peaks in NMR . . . This is the basis of (for example) the $\ce {D2O}$ shake test, and is also the reason why peptide protein NMR is commonly run using $90\%~\ce {H2O} 10\%~\ce {D2O}$ as a solvent (or else the NH peaks would be unobservable) What causes them to disappear from the spectrum?
Convergence issue in Gaussian - Chemistry Stack Exchange Just few suggestions for finding a stationary point You can add some additional angular flexibility into the basis by changing it to, say, 6-31G(2df,p) or even switch to more modern Ahlrichs' def2 bases (Def2SVP to start from) Besides, you'd better use UltraFine integration grid and tight optimization criteria and do geometry optimization and frequency calculation in a single run: Opt=Tight
When to run a blank using ICP-MS - Chemistry Stack Exchange The three blanks required for analysis are the rinse blank, calibration blank, and the laboratory reagent blank My question is, if we assume we are analyzing twenty water samples using ICP-MS, when should each type of blank be run?