General Recommendations for Protocol DNA Electrophoresis • Avoid high salt concentrations in the DNA samples as this may cause bands to shift during electrophoresis • Following electrophoresis, visualize DNA by staining in 0 5 µg ml ethidium bromide solution or SYBR® Green I • Choose the gel percentage according to the tables below: Table 1 Recommended Agarose Gels for Electrophoretic
Section VI: Recovery of DNA from Agarose Gels Recovery of DNA . . . the efficiency of recovery of DNA from agarose gels These tips and recommendations can be applied to all recovery techniques The section is divided into the following topics: — Choosing the appropriate agarose — Choosing the appropriate electrophoresis buffer — Gel casting and DNA loading tips — Staining and recovery tips
Agarose Gel Preparation Protocol - GoldBio Here, we describe a procedure that will help identify and prepare the correct buffer and agarose combination for your experiment, allowing for the most efficient use of your agarose First, determine the best agarose for your application
DNA Restriction Digests and Agarose Gel Electrophoresis Ensure you are using the right buffer, correct ratio of enzyme to substrate (DNA and the right conditions to achieve complete digest) Each restriction enzyme has a buffer in which the highest activity is achieved, usually as a 10X concentrate Some enzymes have common buffers while others need to be used with a unique buffer for optimal activity
Protocol: Preparation of Agarose Gel for DNA Analysis Size-range of DNA fragments to be analyzed: This will help you to determine the agarose percentage in the gel and type of electrophoresis buffer High percentage agarose gel for separation of short DNA fragments and low percentage for separation of large DNA fragments