Assembling the perfect bacterial genome using Oxford . . . Here, we describe a current approach for producing a bacterial genome assembly with the goal of perfection using a combination of Oxford Nanopore Technologies (ONT) long reads and Illumina short reads (Fig 1)
Hybracter: enabling scalable, automated, complete and . . . Complete bacterial genome assembly using hybrid sequencing is a routine and vital part of bacterial genomics, especially for identification of mobile genetic elements and plasmids As sequencing becomes cheaper, easier to access and more accurate, automated assembly methods are crucial
Benchmarking hybrid assembly approaches for genomic . . . Our research demonstrates the hybrid assembly pipeline of Unicycler as a superior approach for genomic analyses of bacterial pathogens using Illumina and Oxford Nanopore sequencing
Hybrid genome assembly - Nanopore and Illumina Learn how to create and assess genome assemblies using the powerful combination of Nanopore and Illumina reads This tutorial explores how long and short read data can be combined to produce a high-quality ‘finished’ bacterial genome sequence
Genome Assembly: Hands-on Training: Hybrid Long Read . . . Understand the concept of hybrid assembly and its advantages in genome sequencing Learn how to perform hybrid assembly using long-read sequencing data from PacBio and ONT platforms
Hybrid Assembly of Bacterial Genome - Arafat Rahman This is genome assembly and annotation workflow that I use for microbial genomics Previously, I used this template to teach different class in OSU as well as in other training facilities
wshropshire flye_hybrid_assembly_pipeline - GitHub Consensus bacterial genome assembly pipeline that utilizes the Flye assembler (https: github com fenderglass Flye) with downstream short-read and long-read correction tools to provide contiguous, circularized replicons with low error rates