Hexokinase 2 senses fructose in tumor-associated . . . - Cell Press Here, we show that a modest amount of fructose, without affecting obesity and associated complications, promotes colorectal cancer tumorigenesis and growth by suppressing the polarization of M1-like macrophages Fructose inhibits M1-like macrophage polarization independently of fructose-mediated metabolism
Isolation and culture of murine bone marrow-derived macrophages for . . . We describe a new and optimised protocol to culture BMDMs We used classically activated macrophages isolated from WT and NO-deficient mice This study emphasises the importance of measuring culture media components and their effects GMCSF priming enhances NO secretion upon inflammatory stimulation