Click-iT EdU Protocol | Flow Cytometry Core | ECU EdU (5-ethynyl-2´-deoxyuridine) is a thymidine analog that is incorporated into DNA during active DNA synthesis Detection is based on click chemistry, which is a copper-catalyzed covalent reaction between an azide and an alkyne In this application, the alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to the Alexa
Click-iT EdU Cell Proliferation Assays - Thermo Fisher Scientific The Invitrogen Click-iT EdU cell proliferation assays combine EdU (5-ethynyl 2´-deoxyuridine) labeling with click chemistry to provide an alternative to traditional BrdU staining methods for detecting and quantitating newly synthesized DNA Click-iT EdU assays simplify the experimental process while providing flexibility to use a variety of fluorescent conjugates
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Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa . . . The Click-iT EdU Alexa Fluor 555 Imaging Kit is a superior alternative to traditional proliferation assays that is optimized for fluorescence microscopy applications In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor dye in a