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- GitHub - deweylab RSEM: RSEM: accurate quantification of gene and . . .
Introduction RSEM is a software package for estimating gene and isoform expression levels from RNA-Seq data The RSEM package provides an user-friendly interface, supports threads for parallel computation of the EM algorithm, single-end and paired-end read data, quality scores, variable-length reads and RSPD estimation
- 【生信】【转录组】RSEM转录本定量 - 知乎
最后，我们将多个样本的gene isoforms的FPKM或者expected_count结果，汇总成矩阵，这个就是后续进行差异分析、富集分析等其他高级分析的输入文件了。 矩阵之美，在于哪？ 至此，RSEM的表达定量已完成，当然，表达量也还有一些小的统计和可视化，如下：
- 用RSEM进行转录本定量分析 | 生信技工
2 RSEM简介 RSEM（RNA-Seq by Expectation-Maximization）是从RNA-Seq数据定量估计基因和转录本表达水平的软件包。 RSEM的特点 RSEM包提供了一个用户友好的界面，支持并行计算EM算法的线程，单端和双端reads，质量评分，长reads读取和RSPD估计。
- RSEM: accurate transcript quantification from RNA-Seq data with or . . .
In this paper, we present a user-friendly software package, RSEM (RNA-Seq by Expectation Maximization), which implements our quantification method and provides extensions to our original model A key feature unique to RSEM is the lack of the requirement of a reference genome
- RSEM完全掌握指南：从原理到落地的实战手册-CSDN博客
RSEM（RNA-Seq by Expectation-Maximization）作为转录组定量领域的经典工具，其核心优势在于创新性地将 期望最大化算法（EM算法） 应用于转录本表达水平估计。 在EM cpp源码中（第314-539行），我们可以清晰看到RSEM如何通过多轮迭代求解转录本丰度：
- RSEM Tutorial: Gene Isoform Quantification for RNA-Seq
Step-by-step RSEM tutorial for RNA-Seq gene and isoform quantification Covers installation, reference preparation, alignment, and interpreting TPM FPKM output
- rsem – Bioinformatics guidance page
RSEM is a software package for estimating gene and isoform expression levels from RNA-Seq data (Li and Dewey 2011) The RSEM package supports threads for parallel computation of the EM algorithm, single-end and paired-end read data, quality scores, variable-length reads and RSPD estimation
- RSEM (RNA-Seq by Expectation-Maximization) - GitHub Pages
Bo Li and Colin Dewey designed the RSEM algorithm Bo Li implemented the RSEM software Peng Liu contributed the STAR aligner options and pRSEM License
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