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- 9: Dilutions and Spectrophotometry - Biology LibreTexts
Based on your absorbance readings, create a standard curve by plotting concentration vs absorbance How well did your data align with Beer’s Law? Explain any deviations If an unknown sample had an absorbance reading of 0 35 at 600 nm, use your standard curve to estimate its concentration
- Lab 16 - Turbidity and Standard Curve Flashcards - Quizlet
How would we determine cell concentration? Through using a hemocytometer What did we do for this lab? -generated a standard curve, so we could estimate concentration based on known absorbance -what is the initial concentration? #2: total dilution factor of 1 -20 cfu on plate of last dilution -what was the initial concentration? a
- 2. 1: Procedure and Data Analysis - Evaluation of Calibration . . .
Give a descriptive title at the top of the figure (eg "Standard curve: Absorbance at 612 nm vs concentration of bromocresol green at pH ~10 ") Give a figure number and a descriptive caption under the figure (eg "Figure 1 The relationship between absorbance at 612 nm and the concentration of bromocresol green in 0 1 M sodium acetate matrix
- ELISA-Why does my diluted high absorbance sample have such . . .
Our lab concluded that the relationship between the absorbance and converted ng ml values broke down when the absorbance reached the upper limits of the standard curve I appreciate all your
- Spectrophotometry Dilutions - UMass
Determine precision and accuracy of dilutions Determine protein concentration with spectrophotometer Pros and cons of conventional spectrophotometer vs 96-well plate absorbance reader
- A two-stage experimental design for dilution assays - PMC
We developed a two-stage experiment with a first stage that obtains an order of magnitude estimate and a second stage that concentrates effort on the most informative dilution to increase estimator precision
- Spectrophotometry Dilutions - Northern Kentucky University
Notice that as concentration increases, absorbance increases as well While you can estimate concentration of an unknown from just looking at the graph, a more accurate way to determine concentration to actually use the equation of the line which fits to your data points
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