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- Ribonuclease III mechanisms of double-stranded RNA cleavage
For example, single-strand cleavage can create hairpin structures at RNA 3′-ends that act as stability determinants 49 Secondary structural elements such as loops, bulges, or mismatches at target sites create deviations from a regular double-helical structure, and would allow only one of the two strands to productively engage a catalytic
- RNA: Two strands are tougher than one | ScienceDaily
Duplex Structure of Double-Stranded RNA Provides Stability against Hydrolysis Relative to Single-Stranded RNA Environmental Science Technology , 2021; 55 (12): 8045 DOI: 10 1021 acs est 1c01255
- RNase A: Frequently Asked Questions - AG Scientific
Recommended working concentration of RNase A is 1 to 100 µg mL depending on the application The enzyme is active under a wide range of reaction conditions At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well as RNA strand in RNA-DNA hybrids
- RNases in Molecular Biology: Roles and Mechanisms
The specificity of RNase III for double-stranded RNA is a key advantage in research, particularly in studies involving RNA interference However, its strong preference for these substrates can limit its application in scenarios involving single-stranded RNA, which researchers must account for in experimental protocols
- Action of nucleases on double-stranded RNA - PubMed
It was found that only the two former enzymes cut across both strands, RNase T1 cannot cleave double strands RNase III was shown to digest double-stranded RNA by a two step process: an initial phase of specific cleavage is followed by random degradation In the first phase the enzyme exhibited a definite preference for some specific base pattern
- Double-stranded RNA - Wikipedia
Double-stranded RNA (dsRNA) is RNA with two complementary strands found in cells It is similar to DNA but with the replacement of thymine by uracil and the adding of one oxygen atom [ 1 ] Despite the structural similarities, much less is known about dsRNA
- RNases - NEB
It can also be used to eliminate single-stranded primers for nested PCR reactions or single-stranded DNA (ssDNA) to leave behind double-stranded DNA (dsDNA) samples Monarch ® RNase A is a component of the Monarch Genomic DNA Purification Kit which degrades single-stranded RNA at C and U residues It is used at 56°C in the Monarch Genomic DNA
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