RNA sequencing data analysis using R and the Artemis HPC The STAR-generated count table is called DATASETNAME_ReadsPerGene out tab We can download it from our HPC server, load it into R, and explore it using standard R libraries
RNA-seq: Explain STAR quantMode geneCounts values - biostars STAR outputs read counts per gene into ReadsPerGene out tab file with 4 columns which correspond to different strandedness options: column 1: gene ID column 2: counts for unstranded RNA-seq column 3: counts for the 1st read strand aligned with RNA (htseq-count option -s yes)
How to merge STAR output reads Counts ReadsPerGene. out. tab? The ReadsPerGene out tab output files of STAR (from option –quantMode GeneCounts) contain 4 columns that correspond to different counts per gene calculated according to the protocol’s strandedness
STAR manual - AdaWongCorner - 博客园 生成两个文件: 1) Aligned toTranscriptome out bam 2) ReadsPerGene out tab PART6 2-pass mapping 为了能准确发现新的剪切位点,力推使用STAR的 2-pass mode。 这并不是说增加检测的新剪切位点的数目,而是增强了检测到可变剪切reads比对到新剪切位点的能力。