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  • dna sequencing - What does chrUn mean in the output from a Bowtie run . . .
    After having performed an alignment using bowtie2 and GRCh38 as a reference sequence, I got inusual matches on chrUn Here a small part of the SAM file: @SQ SN:chr22 LN:50818468 @SQ SN:c
  • metabolism - Why are 6 turns of the Calvin cycle needed to make one . . .
    I‘ve read that 6 turns of the Calvin cycle are required to make 1 glucose molecule But, 3CO 2 and 3RuBP are used in one cycle and 6 triose phosphate is produced Only 5 triose phosphate molecules are required for RuBP regeneration The remaining 1 triose phosphate has 3 carbon atoms in it Only 3 more carbon atoms are needed, which can be produced again in the next cycle So, it would seem
  • Does food continue to stay sequential once it is inside my body?
    In general, food is not kept in any particular sequential order The stomach has a lot of smooth muscle which churns the food, very rapidly erasing any "order" to the food Beyond that, the stomach digests "the food in the stomach," in parallel Whatever is in it, it digests Now, in general, food eaten earlier will digest into chyme (the digested slurry which enters the intestines) sooner
  • fluorescent microscopy - How to set threshold in ImageJ for quantifying . . .
    Presumably your image is either a blot, or of cells You're going to use some software to measure the density of the blots or cells - what software? You can use ImageJ if you haven't another way Either way you need to have the original images in a lossless format for the quantification I think if you adjust levels and click 'apply', you stand to change pixel values I've used an old program


















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