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- Isolation, Purification, and Application of Protoplasts and . . .
In this review, we provide a comprehensive overview, including details on isolation procedures and influencing factors, purification and viability assessment methodologies, and the utilization of the protoplast transient expression system
- Protoplast Isolation- mechanical and enzymatic methods
Mechanically, protoplasts have been isolated by gently teasing the new callus tissue initiated from expanded leaves of Saintpaulia ionantha which was grown in a specific auxin environment to produce thin cell walls However, this method is generally not followed because of certain disadvantages
- Protoplast Culture: Isolation and Culture Methods - Plant . . .
The separated protoplasts, using mechanical or enzymatic techniques are put under the purification process Here, all the undigested cells, undigested tissues, and damaged protoplasts are filtered out
- Protoplast Isolation - an overview | ScienceDirect Topics
Mechanical or enzymatic isolation are the most common approaches to remove the cell wall without damaging the protoplast Mechanical isolation, although possible, often results in low yields, poor quality, and poor performance in culture due to substances released from damaged cells
- Protoplasts Isolation - Biology Notes Online
Klercker (1892) removed protoplasts of Stratiotes aloides using this method Mechanically, protoplasts were removed by gently pulling the new callus tissue arising from the expanded leaves of Saintpaulia Ionantha, which was grown in an auxin-rich environment to create thin cell wall
- Protoplast isolation
Klecker in 1892 has first initiated the protoplast isolation by mechanical means- the cells were kept in a suitable medium plasmolyticum and cut with a fine knife
- Protoplast fusion : Isolation of protoplast - Mechanical and . . .
Mechanical method In this method, cells are kept in a suitable plasmolyticum (protoplast shrink away from cell wall in a plasmolysed cell) and cut with a fine knife, so that protoplasts are released from cells through the opening of the cell wall
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