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- Recognize. Rule-Out. Refer. - APHL
Using automated or manual identification systems (e g , MALDI-TOF, Vitek, API 20 NE, Bactec) may result in exposure to dangerous pathogens, and could result in erroneous identification (e g , Bacillus anthracis misidentified as B cereus; Yersinia pestis misidentified as Y pseudotuberculosis, etc )
- SENTINEL LEVEL CLINICAL LABORATORY GUIDELINES
This procedure describes the steps to rule out, recognize, and presumptively identify this organism in clinical specimens in Sentinel Clinical Laboratories Such laboratories
- Microbiological Laboratory Diagnosis of Human Brucellosis: An Overview . . .
A laboratory diagnosis can be carried out using three different approaches and microbiological procedures: direct diagnosis by culture, indirect diagnosis by serological tests, and rapid diagnosis by molecular PCR-based methods
- Biothreat Preparedness Training: Brucella spp. - CDC
Describe laboratory tests for identification of Brucella spp Describe how to rule out or refer isolates for Brucella spp using standardized laboratory protocols Discuss the role of the sentinel laboratory in bioterrorism response
- Brucella species - New York State Department of Health
Brucellosis is a commonly acquired laboratory infection; all work on suspect Brucella spp cul-tures should be performed at a minimum under BSL2 conditions with BSL3 practices *Oxidase, Urease, and Motility: Appearances of test results are not agent- specific Photos represent typical reactions
- Louisiana Office of Public Health Laboratories
To rule out or confirm bacterial isolates as Brucella spp or to rule out or confirm presence absence of Brucella spp in direct patient specimens Brucella is suspected and cannot be ruled out if the isolate fulfills the
- Brucella culture and genus identification Standard Operating Procedure
isolation and culture of Brucella are usually performed on solid media This is generally the most satisfactory method as it enables the developing colonies to be isolated and recognised clearly Such media also limit the establishment of non-smooth mutants and excessive development of contaminants Sub-culture may be performed on non-selective
- BRUCELLA CULTURE - AAVLD
Cultures that are suspected to be Brucella should only be manipulated under BSL-3 conditions Center for Disease Control (CDC) recommends consultation with state public health laboratory if Brucella spp is suspected in clinical specimens
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