Fluorescence Resonance Energy Transfer (FRET) Microscopy The mechanism of fluorescence resonance energy transfer involves a donor fluorophore in an excited electronic state, which may transfer its excitation energy to a nearby acceptor chromophore in a non-radiative fashion through long-range dipole-dipole interactions The theory supporting energy transfer is based on the concept of treating an excited fluorophore as an oscillating dipole that can
Fluorescence Resonance Energy Transfer - Chemistry LibreTexts Fluorescence Resonance Energy Transfer (FRET) is a special technique to gauge the distance between two chromophores, called a donor-acceptor pair The limitation of FRET is that this transfer process …