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  • Overview of qPCR - NEB
    Script In contrast to traditional PCR where the presence or absence of a DNA sequence is analysed upon reaction completion, quantitative PCR or qPCR, monitors DNA amplification as the reaction progresses In qPCR assays, progression is monitored by an increase in fluorescence signal The fluorophore can be a double stranded DNA binding dye, most commonly SYBR Green 1, or covalently attached to


















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