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- Luciferase - Wikipedia
Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively [1]
- Luciferase | Definition, Bioluminescence, Enzyme, Reaction, Facts . . .
Luciferase, enzyme manufactured in the cells of certain organisms to control bioluminescence The widespread bioluminescence of such living organisms as fireflies and bacteria is based on the oxidation of a luciferin molecule in the presence of the corresponding luciferase
- Luciferase: A Powerful Bioluminescent Research Tool
Luciferase is a group of enzymes that oxidize a substrate known as luciferin to produce light 1 For bioluminescence, the firefly luciferase enzyme catalyzes the oxidation of D-luciferin in the presence of oxygen (O 2), adenosine triphosphate (ATP), and magnesium ions (Mg 2+) This catalytic reaction converts the excited oxyluciferin molecule to its ground state, emitting visible light
- Everything About Luciferin and Luciferase – GoldBio
Luciferase is an enzyme that catalyzes a light-producing biochemical reaction when it is in the presence of oxygen, ATP, magnesium, and a naturally occurring substrate called luciferin Because of the light-emitting phenomenon, the luciferase-luciferin enzyme-substrate combination is the basis behind one of nature’s most common forms of bioluminescence
- Fluorescent Proteins 101: Luciferases - Addgene
Natural and engineered luciferases Although firefly luciferase was the first bioluminescent reporter to be cloned and remains popular, other luciferases have been identified in the sea pansy (Renilla reniformis), click beetle (Pyrophorus plagiophthalamus), deep-sea copepod (Gaussia princeps), Japanese firefly (Luciola cruciata), and more These variant proteins offer different kinetics
- Luciferase Assay: Principles, Purpose, and Process | Ubigene
Essential guide to luciferase assays for gene research Discover purpose, principles, troubleshooting,and protocols for accurate, reliable results
- Luciferase - an overview | ScienceDirect Topics
Luciferase is an enzyme found in fireflies that facilitates the conversion of luciferin to oxyluciferin in the presence of ATP, O2, and Mg2+, resulting in the emission of yellow light It is commonly used to detect low levels of gene expression due to its background-free bioluminescence properties AI generated definition based on: Progress in Neurobiology, 2001
- PDB-101: Molecule of the Month: Luciferase
The cool yellowish light of fireflies is created by the enzyme luciferase, shown here from PDB entry 2d1s The creation of light is not an easy process It requires a lot of energy—a single photon of green light requires about the same energy as the breaking of eight ATP molecules So, luciferase uses a very energetic process to create light It has a cofactor, termed a luciferin, that
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