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- Luciferase - Wikipedia
Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively [1]
- Luciferase | Definition, Bioluminescence, Enzyme, Reaction, Facts . . .
Luciferase, enzyme manufactured in the cells of certain organisms to control bioluminescence The widespread bioluminescence of such living organisms as fireflies and bacteria is based on the oxidation of a luciferin molecule in the presence of the corresponding luciferase
- 技术|Luciferase 报告基因系统方法及原理介绍 - 知乎
荧光素酶报告基因实验（luciferase assay）是检测这类转录因子和其靶启动子中的特异顺序结合的重要手段。 其原理简述如下： （1）构建一个将靶启动子的特定片段插入到荧光素酶表达序列前方的报告基因质粒，如 pGL3-basic 等。 （2） 将要检测的转录因子表达质粒与报告基因质粒共转染 HEK293T细胞 或其它相关的细胞系。 如果此转录因子能够激活靶启动子，则荧光素酶基因就会表达，荧光素酶的表达量与转录因子的作用强度成正比。 （3） 加入特定的荧光素酶底物，荧光素酶与底物反应，产生荧光，通过检测荧光的强度可以测定荧光素酶的活性，从而判断转录因子是否能与此靶启动子片段有作用。 基本实验步骤：
- A Deep Dive Into the Luciferase Assay: What It is, How It Works and . . .
What is a Luciferase Reporter Assay? A luciferase reporter assay is a test that investigates whether a protein can activate or repress the expression of a target gene using luciferase as a reporter protein (Carter Shieh, 2015)
- Luciferase: A Powerful Bioluminescent Research Tool
Luciferase is a group of enzymes that oxidize a substrate known as luciferin to produce light 1 For bioluminescence, the firefly luciferase enzyme catalyzes the oxidation of D-luciferin in the presence of oxygen (O 2), adenosine triphosphate (ATP), and magnesium ions (Mg 2+)
- Luciferase, an alternative to fluorescence - Integrated DNA Technologies
Luciferase enzymes regulate bioluminescence in cells and can be found in a variety of organisms It provides a useful research tool that allows for the study of gene regulation, among other things, and offers an alternative method to fluorescent techniques Check out this DECODED article to find out what it is and how it can be used in the lab
- Fluorescent Proteins 101: Luciferase - Addgene
Learn more about luciferases, a class of enzymes used in bioluminescence, and how they are commonly used in reporter plasmids
- Luciferase | Encyclopedia. com
Luciferase — An enzyme that catalyzes the reaction between oxygen and luciferin Luciferin — Complex carbon molecules that produce light when oxidized Oxidation — The process where a molecule loses one or more electrons
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