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- Isolation of Extracellular Vesicles from Human Follicular Fluid: Size . . .
Follicular fluid (FF) is the microenvironment where a growing oocyte develops Intrafollicular communication ensures oocyte competence and is carried out through paracrine signaling, the exchange of molecules via gap junctions, and the trafficking of extracellular vesicles (EVs)
- Development of a novel protocol for isolation and purification of human . . .
Follicular fluid was collected from patients undergoing oocyte retrieval A series of isolation and purification techniques was performed, involving density gradient centrifugation and use of different antibody-bead complexes
- Protocol for Exosome Isolation from Small Volume of Ovarian Follicular . . .
Here, we describe the process of FF collection from human and bovine and the enrichment and isolation of EVs that we termed folliculosomes (FFEs), using available commercial kits as well as the traditional ultracentrifugation methods The follicular fluid (FF) provides the microenvironment for the developing oocyte [1, 2, 3, 4]
- Diversity of Extracellular Vesicles in Human Follicular Fluid . . .
Isolating EVs from FF where samples are biobanked before research use is challenging Several methods are used to isolate EVs The most commonly used method is UltraCentrifugation (UC) in the field [9]
- Optimization of The Cell Aggregates Method for Isolation and . . .
In assisted reproductive technology, GCs can be collected from follicular fluid (FF) during oocyte retrieval, form women undergoing controlled ovarian stimulation (COS) (5) The GCs in FF may be present as free cells or as clearly visible aggregates (Aggs)
- Protocol for Exosome Isolation from Small Volume of Ovarian Follicular . . .
Here, we describe the process of FF collection from human and bovine and the enrichment and isolation of EVs that we termed folliculosomes (FFEs), using available commercial kits as well as the traditional ultracentrifugation methods
- Follicular fluid exosomes isolated with the different . . . - ResearchGate
In the current study, we investigated the role of dihydrotestosterone-induced exosomal miR-379-5p release in determining the destiny of the developing follicles
- Extracellular microRNA profiling in human follicular fluid: new . . .
In this regard, we summarize the reported miRNAs in follicular fluid and their correlations with female infertility and ART success rate, while subsequent investigations are required Keywords: MicroRNAs, Biomarkers, Reproductive medicine, Follicular fluid, Assisted reproductive techniques
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