Orthogonalized human protease control of secreted signals Engineering of a human-derived protease controlled exogenously by its FDA-approved inhibitor enables control over cytokine activity in cell-based therapies with reduced risk of immunogenicity
Orthogonalized human protease control of secreted signals We sourced a specific human protease and its FDA-approved inhibitor We engineered cytokines (IL-2, IL-6, and IL-10) whose activities can be activated and abrogated by proteolytic cleavage We utilized species specificity and re-localization strategies to orthogonalize the cytokines and protease from the human context that they would be
基于人源蛋白酶的分泌信号正交调控：为细胞疗法带来新曙光 - 生物通 《Nature Chemical Biology》：Orthogonalized human protease control of secreted signals 【字体： 大 中 小 】 时间：2025年01月23日 来源：Nature Chemical Biology 13
Orthogonalized human protease control of secreted signals,bioRxiv . . . We utilized species specificity and re-localization strategies to orthogonalize the cytokines and protease from the human context that they would be deployed in hDIRECT should enable local cytokine activation to support a variety of cell-based therapies such as muscle regeneration and cancer immunotherapy
Orthogonalized human protease control of secreted signals Here we present two genetically encoded orthogonal regulatory secretion systems, which rely on the retention of pre-synthesized proteins on the ER membrane (membER, released by a cytosolic
Orthogonalized human protease control of secreted signals Here, we developed Humanized Drug Induced Regulation of Engineered CyTokines (hDIRECT) as a platform to control cytokine activity exclusively using human-derived proteins We sourced a specific human protease and its FDA-approved inhibitor