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- In vivo genome editing using Staphylococcus aureus Cas9
Here, we characterize six smaller Cas9 orthologues and show that Cas9 from Staphylococcus aureus (SaCas9) can edit the genome with efficiencies similar to those of SpCas9, while being more
- Dynamics of Staphylococcus aureus Cas9 in DNA target Association and . . .
Staphylococcus aureus Cas9 (SaCas9) is an RNA‐guided endonuclease that targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage Its small size facilitates in vivo delivery for genome editing in various organisms
- Characterization of Staphylococcus aureus Cas9: a smaller Cas9 for all . . .
Our results reveal an S aureus Cas9 that is effective for a variety of genome engineering purposes, including paired nickase approaches and all-in-one delivery of Cas9 and multiple gRNA expression cassettes with AAV vectors
- In vivo genome editing using Staphylococcus aureus Cas9
Here, we characterize six smaller Cas9 orthologues and show that Cas9 from Staphylococcus aureus (SaCas9) can edit the genome with efficiencies similar to those of SpCas9, while being more than 1 kilobase shorter
- SaCas9 - Addgene
Plasmid SaCas9 from Dr Isaac Hilton's lab contains the insert Cas9 and is published in ACS Synth Biol 2022 Oct 21;11 (10):3239-3250 doi: 10 1021 acssynbio 2c00156 Epub 2022 Sep 26 This plasmid is available through Addgene
- SaCas9 and Its Role in Precision Genome Editing
SaCas9 enables precise genetic modifications by creating double-strand breaks (DSBs) at predetermined loci, which cells repair through endogenous pathways Non-homologous end joining (NHEJ) often results in insertions or deletions (indels), making it useful for gene disruption, while homology-directed repair (HDR) allows for precise sequence
- Superior Fidelity and Distinct Editing Outcomes of SaCas9 Compared with . . .
SaCas9 is the ideal nuclease for in vivo gene editing due to its small size, high efficiency, and low off-target activities We validated previous reports that 21-nt or 22-nt sgRNAs are optimal for SaCas9 editing
- High-fidelity SaCas9 identified by directional screening in . . . - PLOS
This study characterizes the off-target activity of wild-type SaCas9 and uses a human cell-based screening system to identify high-fidelity SaCas9 variants, showing that they can dramatically reduce off-target effects
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