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- Taq Polymerase - an overview | ScienceDirect Topics
Taq DNA Polymerase Taq DNA polymerase is an 832-amino acid protein with an inferred molecular weight of 93,920 and a specific activity of 292,000 units mg; optimal polymerization activity is achieved at 75–80 ° C, with half-maximal activity at 60–70 ° C (Lawyer et al , 1993; see also Table 1)
- Taq Polymerase - an overview | ScienceDirect Topics
Taq Polymerase is a type of DNA polymerase enzyme commonly used in real-time PCR due to its robust activity and speed It is known for its residual activity at low temperatures, which can lead to non-specific amplification, but 'hot-start' versions have been developed to inhibit its activity at low temperatures and prevent bias in the reaction AI generated definition based on: Biological
- A simple and efficient method for Taq DNA polymerase purification based . . .
Taq DNA polymerase from Thermus aquaticus (Taq) is a widely used enzyme in molecular biology research and clinical diagnostics because of its utility in DNA amplification and sequencing assays For laboratory large-scale production of enzymes, effort, time and cost are crucial factors Here we describe a simple and efficient method for the production of high-quality recombinant Taq DNA
- Taq Polymerase - an overview | ScienceDirect Topics
Taq DNA polymerase The Taq DNA polymerase used in the PCR is the DNA polymerase I enzyme of the bacterium Thermus aquaticus This organism lives in hot springs, and many of its enzymes, including the Taq DNA polymerase, are thermostable Therefore, they are resistant to denaturation by heat treatment
- Taq Polymerase - an overview | ScienceDirect Topics
Taq polymerase is defined as the thermostable DNA polymerase I enzyme derived from the bacterium Thermus aquaticus, which is resistant to heat denaturation, making it suitable for polymerase chain reaction (PCR) applications
- Development of a chemiluminescent detection method for absolute . . .
In our work, we developed a straightforward and precise quantitation approach for Taq DNA polymerase according to chemiluminescence detection of the consumption of dATP (CDC-dATP) throughout complementary polymerization by Taq DNA polymerase along a single-strand circular M13 DNA template he principle of the CDC-dATP method is depicted in Fig 1
- Bst polymerase — a humble relative of Taq polymerase
The biochemical characteristics of the two enzymes and Taq-polymerase were also compared (Table 1) The specific activity, k cat, and processivity of Bst polymerase were significantly higher than those of Taq-polymerase and KF At the same time, The KM values for DNA and dNTP were closer across the different enzymes
- High-level expression of codon-optimized Taq DNA polymerase under the . . .
Instead of full-length Taq polymerase, an N-terminal deletion of Taq polymerase was selected To obtain a high-level expression, we attempted to optimize the codon by reducing the rare codon and GC content, and in a second attempt, we optimized the culture conditions for protein expression
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