SaCas9 TadDE - Addgene Plasmid SaCas9 TadDE from Dr David Liu's lab contains the insert TadDE with SaCas9 and is published in Nat Biotechnol 2022 Nov 10 pii: 10 1038 s41587-022-01533-6 doi: 10 1038 s41587-022-01533-6 This plasmid is available through Addgene
Evolution of an adenine base editor into a small, efficient cytosine . . . We also identified a TadA dual base editor (TadDE) that performs equally efficient cytosine and adenine base editing TadCBEs support single or multiplexed base editing at therapeutically relevant genomic loci in primary human T cells and primary human hematopoietic stem and progenitor cells
High performance TadA-8e derived cytosine and dual base . . . - bioRxiv Excitingly, we show TadDE is a new class of dual base editor where a single deaminase domain promotes simultaneous C-to-T and A-to-G base editing in plant cells Multiplexed base editing is demonstrated in transgenic rice plants with TadCBEa and TadDE
High performance TadA-8e derived cytosine and dual base . . . - PubMed A dual base editor, TadDE, promotes simultaneous C-to-T and A-to-G editing Multiplexed base editing with TadCBEa and TadDE is demonstrated in transgenic rice, with no off-target effects detected by whole genome and transcriptome sequencing, indicating high specificity
Diverse nucleotide substitutions in rice base editing mediated by novel . . . Here, we comprehensively examined the base editing activities of multiple evolved TadA8e variants in rice We found that both TadA-CDd and TadA-E27R N46L achieved more robust C-to-T editing than previously reported hyperactive hAID*D, and TadA-CDd outperformed TadA-E27R N46L