Modes of L929 cell death induced by TNF-alpha and other cytotoxic . . . Our results suggest that L929 cells treated with TNF-alpha alone show nuclear changes and a pattern of DNA fragmentation that are atypical of apoptosis In contrast, our results demonstrate that, when augmented with actinomycin D, TNF-alpha induces classical apoptosis in L929 cells
Induction of Apoptosis in TNF-Treated L929 Cells in the Presence of . . . These results indicate that apoptosis can be induced in TNF-treated L929 cells when the cells are protected from necroptosis, and support the notion that partial activation of caspase-8 in the presence of a caspase inhibitor preferential to caspase-3 suppresses both apoptosis and necroptosis
Establishment of a consistent L929 bioassay system for TNF-alpha . . . TNF-alpha cytotoxicity in LPS-treated, phytomitogen-treated, and cytodifferentiation agent-treated AMC supernatants were analyzed by the L929 bioassay system Our results showed that LPS could induce homogeneous TNF-alpha production by AMC whereas, in addition to TNF-alpha, phytomitogens could also induce other TNF-like factors
MODES OF L929 CELL DEATH INDUCED BY TNF-α AND OTHER . . . - ScienceDirect Our results suggest that L929 cells treated with TNF-α alone show nuclear changes and a pattern of DNA fragmentation that are atypical of apoptosis In contrast, our results demonstrate that, when augmented with actinomycin D, TNF-α induces classical apoptosis in L929 cells
TNF-mediated cytotoxicity of L929 cells: role of staurosporine in . . . The role of protein kinase C (PKC) in tumour necrosis factor (TNF)-mediated cytotoxicity was investigated, using the L929 cell line The PKC activator phorbol-12-myristate 13-acetate (PMA) increased proliferation and inhibited TNF-induced cytotoxicity of L929 cells
Tumor Necrosis Factor-α-induced Cell Killing and Activation of . . . In this study, we investigated the impact of NF-κB activation on TNF-α-mediated cell killing in murine L929 fibrosarcoma cells This widely used cell line is of particular interest because TNF-α-induced cell death occurs without co-apoptotic stimuli, such as cycloheximide or actinomycin D
A cytotoxicity assay for tumor necrosis factor employing a . . . - PubMed Cycloheximide (50 micrograms ml) and actinomycin D (5 micrograms ml) sensitized L929 cells to TNF-induced killing By measuring cell killing early (12 h) and late (30 h), responses proportional to TNF concentration were obtained over a dynamic range of 1 to 5000 pg ml
TNFα-Induced Necroptosis and Autophagy via Supression of the p38–NF-κB . . . Tumor necrosis factor alpha (TNF α) has been reported to induce necroptosis and autophagy, but its mechanisms remain unclear In this study, we found that TNF α significantly induced necroptosis and autophagy in murine fibrosarcoma L929 cells
TNF-induced necroptosis in L929 cells is tightly regulated by multiple . . . CYLD is a RIP1-deubiquitinating enzyme, 11 and we and others previously showed that CYLD repression protects L929 cells from TNF-induced necroptosis, 26, 27 consistent with the cytoprotective role of non-degradative ubiquitination of RIP1