Quantitative Detection of Clostridium perfringens by Real-Time PCR in . . . We developed and tested a real-time (Q-)PCR assay for the species-specific detection of C perfringens that targets the phospholipase C (plc) gene and includes an internal amplification control (IAC), making it possible to identify false-negative results, which are common due to the high level of PCR inhibition by food compounds
Detection of Clostridium perfringens in donor milk at a human . . . - PubMed C perfringens can enter breast milk from contaminated pumping environments or devices, thus causing illness in infants The microbiological testing of DHM in breast milk banks can be accomplished using liquid enrichment, along with anaerobic and aerobic culture, which is of immense significance in …
Recombinase polymerase amplification combined with lateral flow . . . In this study, a combination of recombinase polymerase amplification (RPA) and lateral flow biosensor (LFB) was used to visually detect C perfringens in chicken meat and milk Two sets of primers were designed for the plc gene of C perfringens, and the amplification efficiency and specificity of the primers
Clostridium perfringens - ScienceDirect In this study, we characterized C perfringens isolated from dairy farm herds and associated environments, to examine the molecular ecology and antimicrobial sensitivity of the population Dairy farms included bovine, caprine, or ovine herds, producing milk for fluid milk and or cheese products
Recombinase polymerase amplification combined with lateral flow . . . In this study, a combination of recombinase polymerase amplification (RPA) and lateral flow biosensor (LFB) was used to visually detect C perfringens in chicken meat and milk Methods and results: Two sets of primers were designed for the plc gene of C perfringens, and the amplification efficiency and specificity of the primers
Quantitative Detection of Clostridium perfringens by Real-Time PCR in . . . We developed and tested a real-time C perfringens that targets the phospholipase C (plc) gene and includes an internal amplification control (IAC), making it possible to identify false-negative results, which are com-mon due to the high level of PCR inhibition by food com-pounds