Why does my cell subculture have these black dots like appearance? Add 5ml of media and resuspend by pipetting Add 10ml of media into a new 10cm culture dish Add 1ml of resuspended cells into the culture dish (containing 10ml of media) (1:5 dilution) Check the cells by a microscope Put the culture dish into 37oC, 5% CO2 humidified incubator Check the cells every day, and when cells are 80~90% confluency, do
human evolution - What sort of social structure might *Homo . . . 1 Almost universally, there is little evidence for the social structures of early hominids Such things simply do not survive in the fossil record You may be able to glean some idea of homo floresiensis social structure by researching the societies of hunter gather people who still exist today and have histories going back tens of thousands of
Do lobsters form social hierarchies and is the status in hierarchy . . . Is the marine environment less conducive to the establishment of dominance hierarchy structures, or does this just underline the lack of detailed behavioral information about most marine invertebrates? Which suggests that not much is known about the relationship between serotonin levels in lobsters and the position in the social hierarchy
microbiology - What is the advantage of using starter cultures for . . . 7 I agree that a starter culture would out-comete a contaminant (especially if there is no antibiotic in the media) Another advantage of inoculating with starter culture is that your results concerning plasmid preps or preparing competent cells will be easily reproducible
Do male humans have a mons pubis? - Biology Stack Exchange Wikipedia writes: In human anatomy, and in mammals in general, the mons pubis (also known simply as the mons, and known specifically in females as the mons Venus or mons veneris), is a rounded ma
Why are lions the only social cats? - Biology Stack Exchange Granted, lions are definitely the feline with the strongest social behaviors, and they form the largest social groups It is therefore interesting to think about why that is the case, and what group dynamics and tradeoffs that determine the social behaviours of lions
how to culture mc38 cells - Biology Stack Exchange Edit: Similar questions have been asked before: Textbook lab manual for mammalian cell culture Cell culture reagent consumables suppliers can have good step by step recommendations for how to use their products For example Thermo has some guidelines that you may find helpful Specifically, cell culture maintenance would seem to be helpful These folks also have what superficially look like