Luciferase | Definition, Bioluminescence, Enzyme, Reaction, Facts . . . Luciferase, enzyme manufactured in the cells of certain organisms to control bioluminescence The widespread bioluminescence of such living organisms as fireflies and bacteria is based on the oxidation of a luciferin molecule in the presence of the corresponding luciferase
A Deep Dive Into the Luciferase Assay: What It is, How It Works and . . . What is a Luciferase Reporter Assay? A luciferase reporter assay is a test that investigates whether a protein can activate or repress the expression of a target gene using luciferase as a reporter protein (Carter Shieh, 2015)
Luciferase: A Powerful Bioluminescent Research Tool Luciferase is a group of enzymes that oxidize a substrate known as luciferin to produce light 1 For bioluminescence, the firefly luciferase enzyme catalyzes the oxidation of D-luciferin in the presence of oxygen (O 2), adenosine triphosphate (ATP), and magnesium ions (Mg 2+)
Luciferase - an overview | ScienceDirect Topics Luciferase is an enzyme found in fireflies that facilitates the conversion of luciferin to oxyluciferin in the presence of ATP, O2, and Mg2+, resulting in the emission of yellow light It is commonly used to detect low levels of gene expression due to its background-free bioluminescence properties
Luciferase, an alternative to fluorescence - Integrated DNA Technologies Luciferase enzymes regulate bioluminescence in cells and can be found in a variety of organisms It provides a useful research tool that allows for the study of gene regulation, among other things, and offers an alternative method to fluorescent techniques Check out this DECODED article to find out what it is and how it can be used in the lab
1001 lights: luciferins, luciferases, their mechanisms of . . . - PubMed Bioluminescence (BL) is a spectacular phenomenon involving light emission by live organisms It is caused by the oxidation of a small organic molecule, luciferin, with molecular oxygen, which is catalysed by the enzyme luciferase
Molecule of the Month: Luciferase - RCSB: PDB-101 The chemical trick of bioluminescence has been discovered dozens of times during the evolution of life, by bacteria, by fungi, by sea anemones, by dinoflagellates, and, of course, by fireflies In each case, there is a luciferase protein that uses a luciferin cofactor to trap oxygen